Back to Build/check report for BioC 3.24:   simplified   long
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This page was generated on 2026-04-29 10:01 -0400 (Wed, 29 Apr 2026).

HostnameOSArch (*)R versionInstalled pkgs
nebbiolo2Linux (Ubuntu 24.04.4 LTS)x86_644.6.0 RC (2026-04-17 r89917) -- "Because it was There" 4843
Click on any hostname to see more info about the system (e.g. compilers)      (*) as reported by 'uname -p', except on Windows and Mac OS X

Package 2034/2366HostnameOS / ArchINSTALLBUILDCHECKBUILD BIN
singleCellTK 2.23.0  (landing page)
Joshua David Campbell
Snapshot Date: 2026-04-28 14:49 -0400 (Tue, 28 Apr 2026)
git_url: https://git.bioconductor.org/packages/singleCellTK
git_branch: devel
git_last_commit: 3ac722f
git_last_commit_date: 2026-04-28 09:03:00 -0400 (Tue, 28 Apr 2026)
nebbiolo2Linux (Ubuntu 24.04.4 LTS) / x86_64  OK    OK    WARNINGS  NO, package depends on 'GSVA' which is not available
See other builds for singleCellTK in R Universe.


CHECK results for singleCellTK on nebbiolo2

To the developers/maintainers of the singleCellTK package:
- Allow up to 24 hours (and sometimes 48 hours) for your latest push to git@git.bioconductor.org:packages/singleCellTK.git to reflect on this report. See Troubleshooting Build Report for more information.
- Use the following Renviron settings to reproduce errors and warnings.
- If 'R CMD check' started to fail recently on the Linux builder(s) over a missing dependency, add the missing dependency to 'Suggests:' in your DESCRIPTION file. See Renviron.bioc for more information.

raw results


Summary

Package: singleCellTK
Version: 2.23.0
Command: /home/biocbuild/bbs-3.24-bioc/R/bin/R CMD check --install=check:singleCellTK.install-out.txt --library=/home/biocbuild/bbs-3.24-bioc/R/site-library --timings singleCellTK_2.23.0.tar.gz
StartedAt: 2026-04-29 05:12:29 -0400 (Wed, 29 Apr 2026)
EndedAt: 2026-04-29 05:31:52 -0400 (Wed, 29 Apr 2026)
EllapsedTime: 1163.1 seconds
RetCode: 0
Status:   WARNINGS  
CheckDir: singleCellTK.Rcheck
Warnings: 3

Command output

##############################################################################
##############################################################################
###
### Running command:
###
###   /home/biocbuild/bbs-3.24-bioc/R/bin/R CMD check --install=check:singleCellTK.install-out.txt --library=/home/biocbuild/bbs-3.24-bioc/R/site-library --timings singleCellTK_2.23.0.tar.gz
###
##############################################################################
##############################################################################


* using log directory ‘/home/biocbuild/bbs-3.24-bioc/meat/singleCellTK.Rcheck’
* using R version 4.6.0 RC (2026-04-17 r89917)
* using platform: x86_64-pc-linux-gnu
* R was compiled by
    gcc (Ubuntu 13.3.0-6ubuntu2~24.04.1) 13.3.0
    GNU Fortran (Ubuntu 13.3.0-6ubuntu2~24.04.1) 13.3.0
* running under: Ubuntu 24.04.4 LTS
* using session charset: UTF-8
* current time: 2026-04-29 09:12:30 UTC
* checking for file ‘singleCellTK/DESCRIPTION’ ... OK
* checking extension type ... Package
* this is package ‘singleCellTK’ version ‘2.23.0’
* package encoding: UTF-8
* checking package namespace information ... OK
* checking package dependencies ... INFO
Imports includes 80 non-default packages.
Importing from so many packages makes the package vulnerable to any of
them becoming unavailable.  Move as many as possible to Suggests and
use conditionally.
* checking if this is a source package ... OK
* checking if there is a namespace ... OK
* checking for hidden files and directories ... OK
* checking for portable file names ... OK
* checking for sufficient/correct file permissions ... OK
* checking whether package ‘singleCellTK’ can be installed ... OK
* checking installed package size ... INFO
  installed size is  7.0Mb
  sub-directories of 1Mb or more:
    R         1.0Mb
    extdata   1.6Mb
    shiny     3.0Mb
* checking package directory ... OK
* checking ‘build’ directory ... OK
* checking DESCRIPTION meta-information ... OK
* checking top-level files ... OK
* checking for left-over files ... OK
* checking index information ... OK
* checking package subdirectories ... OK
* checking code files for non-ASCII characters ... OK
* checking R files for syntax errors ... OK
* checking whether the package can be loaded ... OK
* checking whether the package can be loaded with stated dependencies ... OK
* checking whether the package can be unloaded cleanly ... OK
* checking whether the namespace can be loaded with stated dependencies ... OK
* checking whether the namespace can be unloaded cleanly ... OK
* checking loading without being on the library search path ... OK
* checking whether startup messages can be suppressed ... OK
* checking dependencies in R code ... WARNING
Missing or unexported object: 'harmony::HarmonyMatrix'
* checking S3 generic/method consistency ... OK
* checking replacement functions ... OK
* checking foreign function calls ... OK
* checking R code for possible problems ... OK
* checking Rd files ... OK
* checking Rd metadata ... OK
* checking Rd cross-references ... WARNING
Missing link(s) in Rd file 'runHarmony.Rd':
  ‘[harmony]{HarmonyMatrix}’

See section 'Cross-references' in the 'Writing R Extensions' manual.

Found the following Rd file(s) with Rd \link{} targets missing package
anchors:
  dedupRowNames.Rd: SingleCellExperiment-class
  detectCellOutlier.Rd: colData
  diffAbundanceFET.Rd: colData
  downSampleCells.Rd: SingleCellExperiment-class
  downSampleDepth.Rd: SingleCellExperiment-class
  featureIndex.Rd: SummarizedExperiment-class,
    SingleCellExperiment-class
  getBiomarker.Rd: SingleCellExperiment-class
  getDEGTopTable.Rd: SingleCellExperiment-class
  getEnrichRResult.Rd: SingleCellExperiment-class
  getFindMarkerTopTable.Rd: SingleCellExperiment-class
  getGenesetNamesFromCollection.Rd: SingleCellExperiment-class
  getPathwayResultNames.Rd: SingleCellExperiment-class
  getSampleSummaryStatsTable.Rd: SingleCellExperiment-class, assay,
    colData
  getSoupX.Rd: SingleCellExperiment-class
  getTSCANResults.Rd: SingleCellExperiment-class
  getTopHVG.Rd: SingleCellExperiment-class
  importAlevin.Rd: DelayedArray, readMM
  importAnnData.Rd: DelayedArray, readMM
  importBUStools.Rd: readMM
  importCellRanger.Rd: readMM, DelayedArray
  importCellRangerV2Sample.Rd: readMM, DelayedArray
  importCellRangerV3Sample.Rd: readMM, DelayedArray
  importDropEst.Rd: DelayedArray, readMM
  importExampleData.Rd: scRNAseq, Matrix, DelayedArray,
    ReprocessedFluidigmData, ReprocessedAllenData, NestorowaHSCData
  importFromFiles.Rd: readMM, DelayedArray, SingleCellExperiment-class
  importGeneSetsFromCollection.Rd: GeneSetCollection-class,
    SingleCellExperiment-class, GeneSetCollection, GSEABase, metadata
  importGeneSetsFromGMT.Rd: GeneSetCollection-class,
    SingleCellExperiment-class, getGmt, GSEABase, metadata
  importGeneSetsFromList.Rd: GeneSetCollection-class,
    SingleCellExperiment-class, GSEABase, metadata
  importGeneSetsFromMSigDB.Rd: SingleCellExperiment-class, msigdbr,
    GeneSetCollection-class, GSEABase, metadata
  importMitoGeneSet.Rd: SingleCellExperiment-class,
    GeneSetCollection-class, GSEABase, metadata
  importMultipleSources.Rd: DelayedArray
  importOptimus.Rd: readMM, DelayedArray
  importSEQC.Rd: readMM, DelayedArray
  importSTARsolo.Rd: readMM, DelayedArray
  iterateSimulations.Rd: SingleCellExperiment-class
  listSampleSummaryStatsTables.Rd: SingleCellExperiment-class, metadata
  plotBarcodeRankDropsResults.Rd: SingleCellExperiment-class
  plotBarcodeRankScatter.Rd: SingleCellExperiment-class
  plotBatchCorrCompare.Rd: SingleCellExperiment-class
  plotBatchVariance.Rd: SingleCellExperiment-class
  plotBcdsResults.Rd: SingleCellExperiment-class
  plotClusterAbundance.Rd: colData
  plotCxdsResults.Rd: SingleCellExperiment-class
  plotDEGHeatmap.Rd: SingleCellExperiment-class
  plotDEGRegression.Rd: SingleCellExperiment-class
  plotDEGViolin.Rd: SingleCellExperiment-class
  plotDEGVolcano.Rd: SingleCellExperiment-class
  plotDecontXResults.Rd: SingleCellExperiment-class
  plotDoubletFinderResults.Rd: SingleCellExperiment-class
  plotEmptyDropsResults.Rd: SingleCellExperiment-class
  plotEmptyDropsScatter.Rd: SingleCellExperiment-class
  plotEnrichR.Rd: SingleCellExperiment-class
  plotFindMarkerHeatmap.Rd: SingleCellExperiment-class
  plotPCA.Rd: SingleCellExperiment-class
  plotPathway.Rd: SingleCellExperiment-class
  plotRunPerCellQCResults.Rd: SingleCellExperiment-class
  plotSCEBarAssayData.Rd: SingleCellExperiment-class
  plotSCEBarColData.Rd: SingleCellExperiment-class
  plotSCEBatchFeatureMean.Rd: SingleCellExperiment-class
  plotSCEDensity.Rd: SingleCellExperiment-class
  plotSCEDensityAssayData.Rd: SingleCellExperiment-class
  plotSCEDensityColData.Rd: SingleCellExperiment-class
  plotSCEDimReduceColData.Rd: SingleCellExperiment-class
  plotSCEDimReduceFeatures.Rd: SingleCellExperiment-class
  plotSCEHeatmap.Rd: SingleCellExperiment-class
  plotSCEScatter.Rd: SingleCellExperiment-class
  plotSCEViolin.Rd: SingleCellExperiment-class
  plotSCEViolinAssayData.Rd: SingleCellExperiment-class
  plotSCEViolinColData.Rd: SingleCellExperiment-class
  plotScDblFinderResults.Rd: SingleCellExperiment-class
  plotScdsHybridResults.Rd: SingleCellExperiment-class
  plotScrubletResults.Rd: SingleCellExperiment-class
  plotSoupXResults.Rd: SingleCellExperiment-class
  plotTSCANClusterDEG.Rd: SingleCellExperiment-class
  plotTSCANClusterPseudo.Rd: SingleCellExperiment-class
  plotTSCANDimReduceFeatures.Rd: SingleCellExperiment-class
  plotTSCANPseudotimeGenes.Rd: SingleCellExperiment-class
  plotTSCANPseudotimeHeatmap.Rd: SingleCellExperiment-class
  plotTSCANResults.Rd: SingleCellExperiment-class
  plotTSNE.Rd: SingleCellExperiment-class
  plotUMAP.Rd: SingleCellExperiment-class
  readSingleCellMatrix.Rd: DelayedArray
  reportCellQC.Rd: SingleCellExperiment-class
  reportClusterAbundance.Rd: colData
  reportDiffAbundanceFET.Rd: colData
  retrieveSCEIndex.Rd: SingleCellExperiment-class
  runBBKNN.Rd: SingleCellExperiment-class
  runBarcodeRankDrops.Rd: SingleCellExperiment-class, colData
  runBcds.Rd: SingleCellExperiment-class, colData
  runCellQC.Rd: colData
  runComBatSeq.Rd: SingleCellExperiment-class
  runCxds.Rd: SingleCellExperiment-class, colData
  runCxdsBcdsHybrid.Rd: colData
  runDEAnalysis.Rd: SingleCellExperiment-class
  runDecontX.Rd: colData
  runDimReduce.Rd: SingleCellExperiment-class
  runDoubletFinder.Rd: SingleCellExperiment-class
  runDropletQC.Rd: colData
  runEmptyDrops.Rd: SingleCellExperiment-class, colData
  runEnrichR.Rd: SingleCellExperiment-class
  runFastMNN.Rd: SingleCellExperiment-class, BiocParallelParam-class
  runFeatureSelection.Rd: SingleCellExperiment-class
  runFindMarker.Rd: SingleCellExperiment-class
  runGSVA.Rd: SingleCellExperiment-class
  runHarmony.Rd: SingleCellExperiment-class
  runKMeans.Rd: SingleCellExperiment-class, colData
  runLimmaBC.Rd: SingleCellExperiment-class, assay
  runMNNCorrect.Rd: SingleCellExperiment-class, assay,
    BiocParallelParam-class
  runModelGeneVar.Rd: SingleCellExperiment-class
  runPerCellQC.Rd: SingleCellExperiment-class, BiocParallelParam,
    colData
  runSCANORAMA.Rd: SingleCellExperiment-class, assay
  runSCMerge.Rd: SingleCellExperiment-class, colData, assay,
    BiocParallelParam-class
  runScDblFinder.Rd: SingleCellExperiment-class, colData
  runScranSNN.Rd: SingleCellExperiment-class, reducedDim, assay,
    altExp, colData, igraph
  runScrublet.Rd: SingleCellExperiment-class, colData
  runSingleR.Rd: SingleCellExperiment-class
  runSoupX.Rd: SingleCellExperiment-class
  runTSCAN.Rd: SingleCellExperiment-class
  runTSCANClusterDEAnalysis.Rd: SingleCellExperiment-class
  runTSCANDEG.Rd: SingleCellExperiment-class
  runTSNE.Rd: SingleCellExperiment-class
  runUMAP.Rd: SingleCellExperiment-class, BiocParallelParam-class
  runVAM.Rd: SingleCellExperiment-class
  runZINBWaVE.Rd: SingleCellExperiment-class, colData,
    BiocParallelParam-class
  sampleSummaryStats.Rd: SingleCellExperiment-class, assay, colData
  scaterPCA.Rd: SingleCellExperiment-class, BiocParallelParam-class
  scaterlogNormCounts.Rd: logNormCounts
  sctkListGeneSetCollections.Rd: GeneSetCollection-class
  sctkPythonInstallConda.Rd: conda_install, reticulate, conda_create
  sctkPythonInstallVirtualEnv.Rd: virtualenv_install, reticulate,
    virtualenv_create
  selectSCTKConda.Rd: reticulate
  selectSCTKVirtualEnvironment.Rd: reticulate
  setRowNames.Rd: SingleCellExperiment-class
  setSCTKDisplayRow.Rd: SingleCellExperiment-class
  singleCellTK.Rd: SingleCellExperiment-class
  subsetSCECols.Rd: SingleCellExperiment-class
  subsetSCERows.Rd: SingleCellExperiment-class, altExp
  summarizeSCE.Rd: SingleCellExperiment-class
Please provide package anchors for all Rd \link{} targets not in the
package itself and the base packages.
* checking for missing documentation entries ... OK
* checking for code/documentation mismatches ... OK
* checking Rd \usage sections ... OK
* checking Rd contents ... OK
* checking for unstated dependencies in examples ... OK
* checking contents of ‘data’ directory ... OK
* checking data for non-ASCII characters ... OK
* checking data for ASCII and uncompressed saves ... OK
* checking R/sysdata.rda ... OK
* checking files in ‘vignettes’ ... OK
* checking examples ... WARNING
Found the following significant warnings:

  Warning in .local(x, ...) : 'normalizeCounts' is deprecated.
  Warning in .local(x, ...) : 'normalizeCounts' is deprecated.
  Warning in .local(x, ...) : 'pairwiseWilcox' is deprecated.
  Warning in .local(x, ...) : 'pairwiseWilcox' is deprecated.
  Warning in .local(x, ...) : 'pairwiseWilcox' is deprecated.
  Warning in .local(x, ...) : 'aggregateAcrossCells' is deprecated.
  Warning in .local(x, ...) : 'normalizeCounts' is deprecated.
  Warning in .local(x, ...) : 'normalizeCounts' is deprecated.
  Warning in .local(x, ...) : 'aggregateAcrossCells' is deprecated.
  Warning in .local(x, ...) : 'aggregateAcrossCells' is deprecated.
  Warning in .local(x, ...) : 'normalizeCounts' is deprecated.
  Warning in .local(x, ...) : 'pairwiseWilcox' is deprecated.
  Warning in .local(x, ...) : 'pairwiseWilcox' is deprecated.
  Warning in .local(x, ...) : 'pairwiseWilcox' is deprecated.
  Warning in .local(x, ...) : 'normalizeCounts' is deprecated.
  Warning in .local(x, ...) : 'pairwiseWilcox' is deprecated.
  Warning in .local(x, ...) : 'normalizeCounts' is deprecated.
  Warning in .local(x, ...) : 'normalizeCounts' is deprecated.
  Warning in .local(x, ...) : 'pairwiseWilcox' is deprecated.
  Warning in .local(x, ...) : 'pairwiseWilcox' is deprecated.
  Warning in .local(x, ...) : 'normalizeCounts' is deprecated.
  Warning in .local(x, ...) : 'normalizeCounts' is deprecated.
  Warning in .local(x, ...) : 'normalizeCounts' is deprecated.
  Warning in .local(x, ...) : 'librarySizeFactors' is deprecated.
  Warning in .local(x, ...) : 'normalizeCounts' is deprecated.
  Warning in .local(x, ...) : 'aggregateAcrossCells' is deprecated.
  Warning in .local(x, ...) : 'aggregateAcrossCells' is deprecated.
  Warning in .local(x, ...) : 'aggregateAcrossCells' is deprecated.
  Warning in .local(x, ...) : 'aggregateAcrossCells' is deprecated.
  Warning in .local(x, ...) : 'aggregateAcrossCells' is deprecated.
  Warning in .local(x, ...) : 'aggregateAcrossCells' is deprecated.
  Warning in .local(x, ...) : 'aggregateAcrossCells' is deprecated.
  Warning in .local(x, ...) : 'aggregateAcrossCells' is deprecated.
  Warning in .local(x, ...) : 'aggregateAcrossCells' is deprecated.
  Warning in .local(x, ...) : 'aggregateAcrossCells' is deprecated.
  Warning in fitTrendVar(fm, fv, ...) : 'fitTrendVar' is deprecated.
  Warning in .local(x, ...) : 'normalizeCounts' is deprecated.
  Warning in .local(x, ...) : 'aggregateAcrossCells' is deprecated.
  Warning in .local(x, ...) : 'aggregateAcrossCells' is deprecated.
  Warning in .local(x, ...) : 'normalizeCounts' is deprecated.
  Warning in .local(x, ...) : 'normalizeCounts' is deprecated.
  Warning in .local(x, ...) : 'normalizeCounts' is deprecated.
  Warning in .local(x, ...) : 'normalizeCounts' is deprecated.
  Warning in fitTrendVar(fm, fv, ...) : 'fitTrendVar' is deprecated.
  Warning in .local(x, ...) : 'pairwiseWilcox' is deprecated.
  Warning in .local(x, ...) : 'pairwiseWilcox' is deprecated.
  Warning in .local(x, ...) : 'normalizeCounts' is deprecated.
  Warning in .local(x, ...) : 'normalizeCounts' is deprecated.
  Warning in .local(x, ...) : 'normalizeCounts' is deprecated.
  Warning in .local(x, ...) : 'normalizeCounts' is deprecated.
  Warning in .local(x, ...) : 'normalizeCounts' is deprecated.
  Warning in .local(x, ...) : 'sumCountsAcrossCells' is deprecated.
  Warning in .local(x, ...) : 'summarizeAssayByGroup' is deprecated.
  Warning in .local(x, ...) : 'normalizeCounts' is deprecated.
  Warning in fitTrendVar(fm, fv, ...) : 'fitTrendVar' is deprecated.
  Warning in .local(x, ...) : 'normalizeCounts' is deprecated.
  Warning in .local(x, ...) : 'librarySizeFactors' is deprecated.
  Warning in .local(x, ...) : 'aggregateAcrossCells' is deprecated.
  Warning in .local(x, ...) : 'aggregateAcrossCells' is deprecated.
  Warning in .local(x, ...) : 'aggregateAcrossCells' is deprecated.
  Warning in .local(x, ...) : 'normalizeCounts' is deprecated.
  Warning in .local(x, ...) : 'normalizeCounts' is deprecated.
  Warning in .local(x, ...) : 'normalizeCounts' is deprecated.
  Warning in .local(x, ...) : 'normalizeCounts' is deprecated.
  Warning in fitTrendVar(fm, fv, ...) : 'fitTrendVar' is deprecated.
  Warning in .local(x, ...) : 'normalizeCounts' is deprecated.
Deprecated functions may be defunct as soon as of the next release of
R.
See ?Deprecated.
Examples with CPU (user + system) or elapsed time > 5s
                           user system elapsed
plotDoubletFinderResults 36.344  0.603  36.947
runSeuratSCTransform     33.035  0.995  34.361
runDoubletFinder         31.867  0.176  32.044
plotScDblFinderResults   30.467  0.576  28.048
runScDblFinder           16.355  1.090  14.456
plotBatchCorrCompare     12.686  0.632  13.322
importExampleData        10.697  1.264  72.406
plotBcdsResults           8.679  0.487   8.538
plotScdsHybridResults     9.013  0.103   8.469
plotDecontXResults        8.259  0.258   8.517
plotCxdsResults           6.926  0.283   7.208
runDecontX                7.122  0.008   7.131
runUMAP                   6.512  0.235   6.747
plotUMAP                  6.600  0.110   6.710
plotEmptyDropsResults     6.661  0.041   6.702
plotEmptyDropsScatter     6.606  0.063   6.669
runEmptyDrops             6.324  0.013   6.337
detectCellOutlier         5.723  0.137   5.861
plotDEGViolin             5.226  0.127   5.346
getEnrichRResult          0.520  0.058   7.753
runEnrichR                0.499  0.069   5.978
* checking for unstated dependencies in ‘tests’ ... OK
* checking tests ...
  Running ‘spelling.R’
  Running ‘testthat.R’
 OK
* checking for unstated dependencies in vignettes ... OK
* checking package vignettes ... OK
* checking re-building of vignette outputs ... OK
* checking PDF version of manual ... OK
* DONE

Status: 3 WARNINGs
See
  ‘/home/biocbuild/bbs-3.24-bioc/meat/singleCellTK.Rcheck/00check.log’
for details.


Installation output

singleCellTK.Rcheck/00install.out

##############################################################################
##############################################################################
###
### Running command:
###
###   /home/biocbuild/bbs-3.24-bioc/R/bin/R CMD INSTALL singleCellTK
###
##############################################################################
##############################################################################


* installing to library ‘/home/biocbuild/bbs-3.24-bioc/R/site-library’
* installing *source* package ‘singleCellTK’ ...
** this is package ‘singleCellTK’ version ‘2.23.0’
** using staged installation
** R
** data
** exec
** inst
** byte-compile and prepare package for lazy loading
** help
*** installing help indices
** building package indices
** installing vignettes
** testing if installed package can be loaded from temporary location
** testing if installed package can be loaded from final location
** testing if installed package keeps a record of temporary installation path
* DONE (singleCellTK)

Tests output

singleCellTK.Rcheck/tests/spelling.Rout


R version 4.6.0 RC (2026-04-17 r89917) -- "Because it was There"
Copyright (C) 2026 The R Foundation for Statistical Computing
Platform: x86_64-pc-linux-gnu

R is free software and comes with ABSOLUTELY NO WARRANTY.
You are welcome to redistribute it under certain conditions.
Type 'license()' or 'licence()' for distribution details.

R is a collaborative project with many contributors.
Type 'contributors()' for more information and
'citation()' on how to cite R or R packages in publications.

Type 'demo()' for some demos, 'help()' for on-line help, or
'help.start()' for an HTML browser interface to help.
Type 'q()' to quit R.

> if (requireNamespace('spelling', quietly = TRUE))
+   spelling::spell_check_test(vignettes = TRUE, error = FALSE, skip_on_cran = TRUE)
All Done!
> 
> proc.time()
   user  system elapsed 
  0.162   0.022   0.172 

singleCellTK.Rcheck/tests/testthat.Rout


R version 4.6.0 RC (2026-04-17 r89917) -- "Because it was There"
Copyright (C) 2026 The R Foundation for Statistical Computing
Platform: x86_64-pc-linux-gnu

R is free software and comes with ABSOLUTELY NO WARRANTY.
You are welcome to redistribute it under certain conditions.
Type 'license()' or 'licence()' for distribution details.

R is a collaborative project with many contributors.
Type 'contributors()' for more information and
'citation()' on how to cite R or R packages in publications.

Type 'demo()' for some demos, 'help()' for on-line help, or
'help.start()' for an HTML browser interface to help.
Type 'q()' to quit R.

> library(testthat)
> library(singleCellTK)
Loading required package: SummarizedExperiment
Loading required package: MatrixGenerics
Loading required package: matrixStats

Attaching package: 'MatrixGenerics'

The following objects are masked from 'package:matrixStats':

    colAlls, colAnyNAs, colAnys, colAvgsPerRowSet, colCollapse,
    colCounts, colCummaxs, colCummins, colCumprods, colCumsums,
    colDiffs, colIQRDiffs, colIQRs, colLogSumExps, colMadDiffs,
    colMads, colMaxs, colMeans2, colMedians, colMins, colOrderStats,
    colProds, colQuantiles, colRanges, colRanks, colSdDiffs, colSds,
    colSums2, colTabulates, colVarDiffs, colVars, colWeightedMads,
    colWeightedMeans, colWeightedMedians, colWeightedSds,
    colWeightedVars, rowAlls, rowAnyNAs, rowAnys, rowAvgsPerColSet,
    rowCollapse, rowCounts, rowCummaxs, rowCummins, rowCumprods,
    rowCumsums, rowDiffs, rowIQRDiffs, rowIQRs, rowLogSumExps,
    rowMadDiffs, rowMads, rowMaxs, rowMeans2, rowMedians, rowMins,
    rowOrderStats, rowProds, rowQuantiles, rowRanges, rowRanks,
    rowSdDiffs, rowSds, rowSums2, rowTabulates, rowVarDiffs, rowVars,
    rowWeightedMads, rowWeightedMeans, rowWeightedMedians,
    rowWeightedSds, rowWeightedVars

Loading required package: GenomicRanges
Loading required package: stats4
Loading required package: BiocGenerics
Loading required package: generics

Attaching package: 'generics'

The following objects are masked from 'package:base':

    as.difftime, as.factor, as.ordered, intersect, is.element, setdiff,
    setequal, union


Attaching package: 'BiocGenerics'

The following objects are masked from 'package:stats':

    IQR, mad, sd, var, xtabs

The following objects are masked from 'package:base':

    Filter, Find, Map, Position, Reduce, anyDuplicated, aperm, append,
    as.data.frame, basename, cbind, colnames, dirname, do.call,
    duplicated, eval, evalq, get, grep, grepl, is.unsorted, lapply,
    mapply, match, mget, order, paste, pmax, pmax.int, pmin, pmin.int,
    rank, rbind, rownames, sapply, saveRDS, table, tapply, unique,
    unsplit, which.max, which.min

Loading required package: S4Vectors

Attaching package: 'S4Vectors'

The following object is masked from 'package:utils':

    findMatches

The following objects are masked from 'package:base':

    I, expand.grid, unname

Loading required package: IRanges
Loading required package: Seqinfo
Loading required package: Biobase
Welcome to Bioconductor

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    'browseVignettes()'. To cite Bioconductor, see
    'citation("Biobase")', and for packages 'citation("pkgname")'.


Attaching package: 'Biobase'

The following object is masked from 'package:MatrixGenerics':

    rowMedians

The following objects are masked from 'package:matrixStats':

    anyMissing, rowMedians

Loading required package: SingleCellExperiment
Loading required package: DelayedArray
Loading required package: Matrix

Attaching package: 'Matrix'

The following object is masked from 'package:S4Vectors':

    expand

Loading required package: S4Arrays
Loading required package: abind

Attaching package: 'S4Arrays'

The following object is masked from 'package:abind':

    abind

The following object is masked from 'package:base':

    rowsum

Loading required package: SparseArray

Attaching package: 'DelayedArray'

The following objects are masked from 'package:base':

    apply, scale, sweep


Attaching package: 'singleCellTK'

The following object is masked from 'package:BiocGenerics':

    plotPCA

> 
> test_check("singleCellTK")
Found 2 batches
Using null model in ComBat-seq.
Adjusting for 0 covariate(s) or covariate level(s)
Estimating dispersions
Fitting the GLM model
Shrinkage off - using GLM estimates for parameters
Adjusting the data
Found 2 batches
Using null model in ComBat-seq.
Adjusting for 1 covariate(s) or covariate level(s)
Estimating dispersions
Fitting the GLM model
Shrinkage off - using GLM estimates for parameters
Adjusting the data
Performing log-normalization
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[----|----|----|----|----|----|----|----|----|----|
**************************************************|

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Calculating gene variances
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[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Calculating feature variances of standardized and clipped values
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[----|----|----|----|----|----|----|----|----|----|
**************************************************|

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Calculating gene variances
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[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Calculating feature variances of standardized and clipped values
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[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Uploading data to Enrichr... Done.
  Querying HDSigDB_Human_2021... Done.
Parsing results... Done.
Performing log-normalization
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[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Calculating gene variances
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[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Calculating feature variances of standardized and clipped values
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[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Calculating gene means
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[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Calculating gene variance to mean ratios
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[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Calculating gene means
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[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Calculating gene variance to mean ratios
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[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Performing log-normalization
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[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Calculating gene variances
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Calculating feature variances of standardized and clipped values
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[----|----|----|----|----|----|----|----|----|----|
**************************************************|

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[05:29:44] WARNING: src/learner.cc:782: 
Parameters: { "nthreads" } are not used.

[05:29:45] WARNING: src/learner.cc:782: 
Parameters: { "nthreads" } are not used.

Performing log-normalization
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[----|----|----|----|----|----|----|----|----|----|
**************************************************|

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  |======================================================================| 100%
Calculating gene variances
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Calculating feature variances of standardized and clipped values
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|

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Modularity Optimizer version 1.3.0 by Ludo Waltman and Nees Jan van Eck

Number of nodes: 390
Number of edges: 9849

Running Louvain algorithm...
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[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Maximum modularity in 10 random starts: 0.8351
Number of communities: 7
Elapsed time: 0 seconds
Using method 'umap'
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[----|----|----|----|----|----|----|----|----|----|
**************************************************|

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[----|----|----|----|----|----|----|----|----|----|
**************************************************|
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[----|----|----|----|----|----|----|----|----|----|
**************************************************|

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Performing log-normalization
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[----|----|----|----|----|----|----|----|----|----|
**************************************************|
[ FAIL 0 | WARN 84 | SKIP 0 | PASS 225 ]

[ FAIL 0 | WARN 84 | SKIP 0 | PASS 225 ]
> 
> proc.time()
   user  system elapsed 
269.752  12.922 409.648 

Example timings

singleCellTK.Rcheck/singleCellTK-Ex.timings

nameusersystemelapsed
MitoGenes0.0020.0010.002
SEG0.0010.0010.003
calcEffectSizes0.1500.0050.155
combineSCE0.7400.0930.832
computeZScore0.2620.0170.280
convertSCEToSeurat4.5310.2544.785
convertSeuratToSCE0.3010.0020.303
dedupRowNames0.0560.0000.056
detectCellOutlier5.7230.1375.861
diffAbundanceFET0.0500.0030.053
discreteColorPalette0.0050.0000.006
distinctColors0.0010.0000.002
downSampleCells0.5130.0180.530
downSampleDepth0.4090.0050.413
expData-ANY-character-method0.1210.0080.128
expData-set-ANY-character-CharacterOrNullOrMissing-logical-method0.1540.0070.161
expData-set0.1460.0060.151
expData0.1180.0020.120
expDataNames-ANY-method0.1170.0000.118
expDataNames0.1110.0010.112
expDeleteDataTag0.0290.0030.032
expSetDataTag0.0230.0000.023
expTaggedData0.0240.0000.024
exportSCE0.0190.0020.022
exportSCEtoAnnData0.0940.0030.097
exportSCEtoFlatFile0.0870.0090.096
featureIndex0.0360.0000.036
generateSimulatedData0.050.000.05
getBiomarker0.0580.0000.058
getDEGTopTable0.6920.0630.757
getDiffAbundanceResults0.0460.0000.046
getEnrichRResult0.5200.0587.753
getFindMarkerTopTable1.5960.0981.694
getMSigDBTable0.0040.0090.013
getPathwayResultNames0.0210.0020.023
getSampleSummaryStatsTable0.2640.0190.283
getSoupX000
getTSCANResults1.0920.1161.208
getTopHVG0.7570.0760.832
importAnnData0.0010.0000.002
importBUStools0.1430.0150.158
importCellRanger0.7390.0660.806
importCellRangerV2Sample0.1360.0060.142
importCellRangerV3Sample0.2890.0130.302
importDropEst0.1890.0040.193
importExampleData10.697 1.26472.406
importGeneSetsFromCollection0.0720.0020.075
importGeneSetsFromGMT0.0650.0130.079
importGeneSetsFromList0.1320.0030.136
importGeneSetsFromMSigDB2.3820.2662.649
importMitoGeneSet0.0510.0000.051
importOptimus0.0010.0000.001
importSEQC0.1060.0010.107
importSTARsolo0.1390.0000.139
iterateSimulations0.1660.0080.174
listSampleSummaryStatsTables0.3030.0010.304
mergeSCEColData0.3420.0080.351
mouseBrainSubsetSCE0.0350.0000.035
msigdb_table0.0010.0000.001
plotBarcodeRankDropsResults0.8950.0180.915
plotBarcodeRankScatter0.7870.0230.811
plotBatchCorrCompare12.686 0.63213.322
plotBatchVariance0.4310.0980.530
plotBcdsResults8.6790.4878.538
plotBubble0.8210.0020.823
plotClusterAbundance1.3260.0231.349
plotCxdsResults6.9260.2837.208
plotDEGHeatmap2.1210.0892.211
plotDEGRegression4.0830.0584.135
plotDEGViolin5.2260.1275.346
plotDEGVolcano0.9160.0370.954
plotDecontXResults8.2590.2588.517
plotDimRed0.2720.0030.274
plotDoubletFinderResults36.344 0.60336.947
plotEmptyDropsResults6.6610.0416.702
plotEmptyDropsScatter6.6060.0636.669
plotFindMarkerHeatmap3.7270.0813.808
plotMASTThresholdGenes1.1920.0291.221
plotPCA0.3630.0030.366
plotPathway0.6630.0050.667
plotRunPerCellQCResults2.9740.0252.998
plotSCEBarAssayData0.2710.0010.272
plotSCEBarColData0.2330.0100.243
plotSCEBatchFeatureMean0.3810.0000.381
plotSCEDensity0.30.00.3
plotSCEDensityAssayData0.2840.0000.285
plotSCEDensityColData0.2950.0010.296
plotSCEDimReduceColData0.7110.0010.713
plotSCEDimReduceFeatures0.3730.0010.374
plotSCEHeatmap0.4250.0030.427
plotSCEScatter0.3540.0270.381
plotSCEViolin0.3580.0100.368
plotSCEViolinAssayData0.3800.0040.384
plotSCEViolinColData0.3750.0170.392
plotScDblFinderResults30.467 0.57628.048
plotScanpyDotPlot0.0200.0010.021
plotScanpyEmbedding0.0210.0000.021
plotScanpyHVG0.0210.0000.021
plotScanpyHeatmap0.0210.0000.021
plotScanpyMarkerGenes0.0210.0000.021
plotScanpyMarkerGenesDotPlot0.0210.0000.021
plotScanpyMarkerGenesHeatmap0.0200.0010.021
plotScanpyMarkerGenesMatrixPlot0.0220.0000.022
plotScanpyMarkerGenesViolin0.0210.0000.021
plotScanpyMatrixPlot0.0200.0010.021
plotScanpyPCA0.0220.0000.022
plotScanpyPCAGeneRanking0.0200.0020.022
plotScanpyPCAVariance0.0210.0000.021
plotScanpyViolin0.0210.0000.021
plotScdsHybridResults9.0130.1038.469
plotScrubletResults0.0220.0010.024
plotSeuratElbow0.0220.0000.022
plotSeuratHVG0.0210.0000.021
plotSeuratJackStraw0.0220.0000.022
plotSeuratReduction0.0220.0000.022
plotSoupXResults000
plotTSCANClusterDEG4.9180.0064.925
plotTSCANClusterPseudo1.3480.0051.354
plotTSCANDimReduceFeatures1.3240.0021.326
plotTSCANPseudotimeGenes1.6140.0151.629
plotTSCANPseudotimeHeatmap1.3330.0041.337
plotTSCANResults1.2080.0051.214
plotTSNE0.4020.0010.403
plotTopHVG0.6090.0000.608
plotUMAP6.600.116.71
readSingleCellMatrix0.0040.0010.005
reportCellQC0.0790.0000.079
reportDropletQC0.0220.0000.022
reportQCTool0.0840.0000.085
retrieveSCEIndex0.0280.0010.030
runBBKNN000
runBarcodeRankDrops0.2340.0000.234
runBcds1.4620.0810.959
runCellQC0.0800.0000.079
runClusterSummaryMetrics0.3820.0030.384
runComBatSeq0.4560.0010.457
runCxds0.2980.0020.301
runCxdsBcdsHybrid1.5120.0630.988
runDEAnalysis0.3850.0000.385
runDecontX7.1220.0087.131
runDimReduce0.2780.0010.279
runDoubletFinder31.867 0.17632.044
runDropletQC0.0210.0010.021
runEmptyDrops6.3240.0136.337
runEnrichR0.4990.0695.978
runFastMNN1.6570.1791.837
runFeatureSelection0.2080.0020.211
runFindMarker1.4670.0811.549
runGSVA0.7190.0720.791
runHarmony0.0390.0040.043
runKMeans0.2050.0530.259
runLimmaBC0.0840.0030.087
runMNNCorrect0.410.030.44
runModelGeneVar0.2990.0250.324
runNormalization2.3650.4042.769
runPerCellQC0.3500.0180.368
runSCANORAMA000
runSCMerge0.0050.0000.004
runScDblFinder16.355 1.09014.456
runScanpyFindClusters0.0220.0000.022
runScanpyFindHVG0.0210.0000.021
runScanpyFindMarkers0.0200.0020.022
runScanpyNormalizeData0.0940.0000.095
runScanpyPCA0.0220.0000.022
runScanpyScaleData0.0210.0000.022
runScanpyTSNE0.0220.0000.022
runScanpyUMAP0.0210.0010.023
runScranSNN0.2830.0030.286
runScrublet0.0210.0000.022
runSeuratFindClusters0.0220.0000.022
runSeuratFindHVG0.4630.0150.479
runSeuratHeatmap0.0230.0000.023
runSeuratICA0.0210.0010.021
runSeuratJackStraw0.0240.0000.023
runSeuratNormalizeData0.0230.0000.022
runSeuratPCA0.0210.0000.022
runSeuratSCTransform33.035 0.99534.361
runSeuratScaleData0.0230.0000.023
runSeuratUMAP0.0220.0010.023
runSingleR0.0390.0000.039
runSoupX000
runTSCAN0.6560.0130.669
runTSCANClusterDEAnalysis0.8260.0290.855
runTSCANDEG0.7110.0030.714
runTSNE0.7190.0100.729
runUMAP6.5120.2356.747
runVAM0.2990.0110.310
runZINBWaVE0.0040.0010.004
sampleSummaryStats0.1550.0120.168
scaterCPM0.1400.0110.151
scaterPCA0.4320.0030.436
scaterlogNormCounts0.2320.0070.239
sce0.0200.0010.021
sctkListGeneSetCollections0.0760.0040.079
sctkPythonInstallConda000
sctkPythonInstallVirtualEnv000
selectSCTKConda000
selectSCTKVirtualEnvironment000
setRowNames0.0830.0000.084
setSCTKDisplayRow0.4190.0140.434
singleCellTK000
subDiffEx0.3370.0030.339
subsetSCECols0.080.000.08
subsetSCERows0.2100.0120.221
summarizeSCE0.0670.0010.067
trimCounts0.1990.0050.204