Back to Build/check report for BioC 3.24:   simplified   long
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This page was generated on 2026-05-13 11:33 -0400 (Wed, 13 May 2026).

HostnameOSArch (*)R versionInstalled pkgs
nebbiolo2Linux (Ubuntu 24.04.4 LTS)x86_644.6.0 RC (2026-04-17 r89917) -- "Because it was There" 4893
Click on any hostname to see more info about the system (e.g. compilers)      (*) as reported by 'uname -p', except on Windows and Mac OS X

Package 2042/2374HostnameOS / ArchINSTALLBUILDCHECKBUILD BIN
singleCellTK 2.23.0  (landing page)
Joshua David Campbell
Snapshot Date: 2026-05-12 13:45 -0400 (Tue, 12 May 2026)
git_url: https://git.bioconductor.org/packages/singleCellTK
git_branch: devel
git_last_commit: 3ac722f
git_last_commit_date: 2026-04-28 09:03:00 -0400 (Tue, 28 Apr 2026)
nebbiolo2Linux (Ubuntu 24.04.4 LTS) / x86_64  OK    OK    WARNINGS  UNNEEDED, same version is already published
See other builds for singleCellTK in R Universe.


CHECK results for singleCellTK on nebbiolo2

To the developers/maintainers of the singleCellTK package:
- Allow up to 24 hours (and sometimes 48 hours) for your latest push to git@git.bioconductor.org:packages/singleCellTK.git to reflect on this report. See Troubleshooting Build Report for more information.
- Use the following Renviron settings to reproduce errors and warnings.
- If 'R CMD check' started to fail recently on the Linux builder(s) over a missing dependency, add the missing dependency to 'Suggests:' in your DESCRIPTION file. See Renviron.bioc for more information.

raw results


Summary

Package: singleCellTK
Version: 2.23.0
Command: /home/biocbuild/bbs-3.24-bioc/R/bin/R CMD check --install=check:singleCellTK.install-out.txt --library=/home/biocbuild/bbs-3.24-bioc/R/site-library --timings singleCellTK_2.23.0.tar.gz
StartedAt: 2026-05-13 04:43:15 -0400 (Wed, 13 May 2026)
EndedAt: 2026-05-13 04:59:56 -0400 (Wed, 13 May 2026)
EllapsedTime: 1000.9 seconds
RetCode: 0
Status:   WARNINGS  
CheckDir: singleCellTK.Rcheck
Warnings: 3

Command output

##############################################################################
##############################################################################
###
### Running command:
###
###   /home/biocbuild/bbs-3.24-bioc/R/bin/R CMD check --install=check:singleCellTK.install-out.txt --library=/home/biocbuild/bbs-3.24-bioc/R/site-library --timings singleCellTK_2.23.0.tar.gz
###
##############################################################################
##############################################################################


* using log directory ‘/home/biocbuild/bbs-3.24-bioc/meat/singleCellTK.Rcheck’
* using R version 4.6.0 RC (2026-04-17 r89917)
* using platform: x86_64-pc-linux-gnu
* R was compiled by
    gcc (Ubuntu 13.3.0-6ubuntu2~24.04.1) 13.3.0
    GNU Fortran (Ubuntu 13.3.0-6ubuntu2~24.04.1) 13.3.0
* running under: Ubuntu 24.04.4 LTS
* using session charset: UTF-8
* current time: 2026-05-13 08:43:16 UTC
* checking for file ‘singleCellTK/DESCRIPTION’ ... OK
* checking extension type ... Package
* this is package ‘singleCellTK’ version ‘2.23.0’
* package encoding: UTF-8
* checking package namespace information ... OK
* checking package dependencies ... INFO
Imports includes 80 non-default packages.
Importing from so many packages makes the package vulnerable to any of
them becoming unavailable.  Move as many as possible to Suggests and
use conditionally.
* checking if this is a source package ... OK
* checking if there is a namespace ... OK
* checking for hidden files and directories ... OK
* checking for portable file names ... OK
* checking for sufficient/correct file permissions ... OK
* checking whether package ‘singleCellTK’ can be installed ... OK
* checking installed package size ... INFO
  installed size is  7.0Mb
  sub-directories of 1Mb or more:
    R         1.0Mb
    extdata   1.6Mb
    shiny     3.0Mb
* checking package directory ... OK
* checking ‘build’ directory ... OK
* checking DESCRIPTION meta-information ... OK
* checking top-level files ... OK
* checking for left-over files ... OK
* checking index information ... OK
* checking package subdirectories ... OK
* checking code files for non-ASCII characters ... OK
* checking R files for syntax errors ... OK
* checking whether the package can be loaded ... OK
* checking whether the package can be loaded with stated dependencies ... OK
* checking whether the package can be unloaded cleanly ... OK
* checking whether the namespace can be loaded with stated dependencies ... OK
* checking whether the namespace can be unloaded cleanly ... OK
* checking loading without being on the library search path ... OK
* checking whether startup messages can be suppressed ... OK
* checking dependencies in R code ... WARNING
Missing or unexported object: 'harmony::HarmonyMatrix'
* checking S3 generic/method consistency ... OK
* checking replacement functions ... OK
* checking foreign function calls ... OK
* checking R code for possible problems ... OK
* checking Rd files ... OK
* checking Rd metadata ... OK
* checking Rd cross-references ... WARNING
Missing link(s) in Rd file 'runHarmony.Rd':
  ‘[harmony]{HarmonyMatrix}’

See section 'Cross-references' in the 'Writing R Extensions' manual.

Found the following Rd file(s) with Rd \link{} targets missing package
anchors:
  dedupRowNames.Rd: SingleCellExperiment-class
  detectCellOutlier.Rd: colData
  diffAbundanceFET.Rd: colData
  downSampleCells.Rd: SingleCellExperiment-class
  downSampleDepth.Rd: SingleCellExperiment-class
  featureIndex.Rd: SummarizedExperiment-class,
    SingleCellExperiment-class
  getBiomarker.Rd: SingleCellExperiment-class
  getDEGTopTable.Rd: SingleCellExperiment-class
  getEnrichRResult.Rd: SingleCellExperiment-class
  getFindMarkerTopTable.Rd: SingleCellExperiment-class
  getGenesetNamesFromCollection.Rd: SingleCellExperiment-class
  getPathwayResultNames.Rd: SingleCellExperiment-class
  getSampleSummaryStatsTable.Rd: SingleCellExperiment-class, assay,
    colData
  getSoupX.Rd: SingleCellExperiment-class
  getTSCANResults.Rd: SingleCellExperiment-class
  getTopHVG.Rd: SingleCellExperiment-class
  importAlevin.Rd: DelayedArray, readMM
  importAnnData.Rd: DelayedArray, readMM
  importBUStools.Rd: readMM
  importCellRanger.Rd: readMM, DelayedArray
  importCellRangerV2Sample.Rd: readMM, DelayedArray
  importCellRangerV3Sample.Rd: readMM, DelayedArray
  importDropEst.Rd: DelayedArray, readMM
  importExampleData.Rd: scRNAseq, Matrix, DelayedArray,
    ReprocessedFluidigmData, ReprocessedAllenData, NestorowaHSCData
  importFromFiles.Rd: readMM, DelayedArray, SingleCellExperiment-class
  importGeneSetsFromCollection.Rd: GeneSetCollection-class,
    SingleCellExperiment-class, GeneSetCollection, GSEABase, metadata
  importGeneSetsFromGMT.Rd: GeneSetCollection-class,
    SingleCellExperiment-class, getGmt, GSEABase, metadata
  importGeneSetsFromList.Rd: GeneSetCollection-class,
    SingleCellExperiment-class, GSEABase, metadata
  importGeneSetsFromMSigDB.Rd: SingleCellExperiment-class, msigdbr,
    GeneSetCollection-class, GSEABase, metadata
  importMitoGeneSet.Rd: SingleCellExperiment-class,
    GeneSetCollection-class, GSEABase, metadata
  importMultipleSources.Rd: DelayedArray
  importOptimus.Rd: readMM, DelayedArray
  importSEQC.Rd: readMM, DelayedArray
  importSTARsolo.Rd: readMM, DelayedArray
  iterateSimulations.Rd: SingleCellExperiment-class
  listSampleSummaryStatsTables.Rd: SingleCellExperiment-class, metadata
  plotBarcodeRankDropsResults.Rd: SingleCellExperiment-class
  plotBarcodeRankScatter.Rd: SingleCellExperiment-class
  plotBatchCorrCompare.Rd: SingleCellExperiment-class
  plotBatchVariance.Rd: SingleCellExperiment-class
  plotBcdsResults.Rd: SingleCellExperiment-class
  plotClusterAbundance.Rd: colData
  plotCxdsResults.Rd: SingleCellExperiment-class
  plotDEGHeatmap.Rd: SingleCellExperiment-class
  plotDEGRegression.Rd: SingleCellExperiment-class
  plotDEGViolin.Rd: SingleCellExperiment-class
  plotDEGVolcano.Rd: SingleCellExperiment-class
  plotDecontXResults.Rd: SingleCellExperiment-class
  plotDoubletFinderResults.Rd: SingleCellExperiment-class
  plotEmptyDropsResults.Rd: SingleCellExperiment-class
  plotEmptyDropsScatter.Rd: SingleCellExperiment-class
  plotEnrichR.Rd: SingleCellExperiment-class
  plotFindMarkerHeatmap.Rd: SingleCellExperiment-class
  plotPCA.Rd: SingleCellExperiment-class
  plotPathway.Rd: SingleCellExperiment-class
  plotRunPerCellQCResults.Rd: SingleCellExperiment-class
  plotSCEBarAssayData.Rd: SingleCellExperiment-class
  plotSCEBarColData.Rd: SingleCellExperiment-class
  plotSCEBatchFeatureMean.Rd: SingleCellExperiment-class
  plotSCEDensity.Rd: SingleCellExperiment-class
  plotSCEDensityAssayData.Rd: SingleCellExperiment-class
  plotSCEDensityColData.Rd: SingleCellExperiment-class
  plotSCEDimReduceColData.Rd: SingleCellExperiment-class
  plotSCEDimReduceFeatures.Rd: SingleCellExperiment-class
  plotSCEHeatmap.Rd: SingleCellExperiment-class
  plotSCEScatter.Rd: SingleCellExperiment-class
  plotSCEViolin.Rd: SingleCellExperiment-class
  plotSCEViolinAssayData.Rd: SingleCellExperiment-class
  plotSCEViolinColData.Rd: SingleCellExperiment-class
  plotScDblFinderResults.Rd: SingleCellExperiment-class
  plotScdsHybridResults.Rd: SingleCellExperiment-class
  plotScrubletResults.Rd: SingleCellExperiment-class
  plotSoupXResults.Rd: SingleCellExperiment-class
  plotTSCANClusterDEG.Rd: SingleCellExperiment-class
  plotTSCANClusterPseudo.Rd: SingleCellExperiment-class
  plotTSCANDimReduceFeatures.Rd: SingleCellExperiment-class
  plotTSCANPseudotimeGenes.Rd: SingleCellExperiment-class
  plotTSCANPseudotimeHeatmap.Rd: SingleCellExperiment-class
  plotTSCANResults.Rd: SingleCellExperiment-class
  plotTSNE.Rd: SingleCellExperiment-class
  plotUMAP.Rd: SingleCellExperiment-class
  readSingleCellMatrix.Rd: DelayedArray
  reportCellQC.Rd: SingleCellExperiment-class
  reportClusterAbundance.Rd: colData
  reportDiffAbundanceFET.Rd: colData
  retrieveSCEIndex.Rd: SingleCellExperiment-class
  runBBKNN.Rd: SingleCellExperiment-class
  runBarcodeRankDrops.Rd: SingleCellExperiment-class, colData
  runBcds.Rd: SingleCellExperiment-class, colData
  runCellQC.Rd: colData
  runComBatSeq.Rd: SingleCellExperiment-class
  runCxds.Rd: SingleCellExperiment-class, colData
  runCxdsBcdsHybrid.Rd: colData
  runDEAnalysis.Rd: SingleCellExperiment-class
  runDecontX.Rd: colData
  runDimReduce.Rd: SingleCellExperiment-class
  runDoubletFinder.Rd: SingleCellExperiment-class
  runDropletQC.Rd: colData
  runEmptyDrops.Rd: SingleCellExperiment-class, colData
  runEnrichR.Rd: SingleCellExperiment-class
  runFastMNN.Rd: SingleCellExperiment-class, BiocParallelParam-class
  runFeatureSelection.Rd: SingleCellExperiment-class
  runFindMarker.Rd: SingleCellExperiment-class
  runGSVA.Rd: SingleCellExperiment-class
  runHarmony.Rd: SingleCellExperiment-class
  runKMeans.Rd: SingleCellExperiment-class, colData
  runLimmaBC.Rd: SingleCellExperiment-class, assay
  runMNNCorrect.Rd: SingleCellExperiment-class, assay,
    BiocParallelParam-class
  runModelGeneVar.Rd: SingleCellExperiment-class
  runPerCellQC.Rd: SingleCellExperiment-class, BiocParallelParam,
    colData
  runSCANORAMA.Rd: SingleCellExperiment-class, assay
  runSCMerge.Rd: SingleCellExperiment-class, colData, assay,
    BiocParallelParam-class
  runScDblFinder.Rd: SingleCellExperiment-class, colData
  runScranSNN.Rd: SingleCellExperiment-class, reducedDim, assay,
    altExp, colData, igraph
  runScrublet.Rd: SingleCellExperiment-class, colData
  runSingleR.Rd: SingleCellExperiment-class
  runSoupX.Rd: SingleCellExperiment-class
  runTSCAN.Rd: SingleCellExperiment-class
  runTSCANClusterDEAnalysis.Rd: SingleCellExperiment-class
  runTSCANDEG.Rd: SingleCellExperiment-class
  runTSNE.Rd: SingleCellExperiment-class
  runUMAP.Rd: SingleCellExperiment-class, BiocParallelParam-class
  runVAM.Rd: SingleCellExperiment-class
  runZINBWaVE.Rd: SingleCellExperiment-class, colData,
    BiocParallelParam-class
  sampleSummaryStats.Rd: SingleCellExperiment-class, assay, colData
  scaterPCA.Rd: SingleCellExperiment-class, BiocParallelParam-class
  scaterlogNormCounts.Rd: logNormCounts
  sctkListGeneSetCollections.Rd: GeneSetCollection-class
  sctkPythonInstallConda.Rd: conda_install, reticulate, conda_create
  sctkPythonInstallVirtualEnv.Rd: virtualenv_install, reticulate,
    virtualenv_create
  selectSCTKConda.Rd: reticulate
  selectSCTKVirtualEnvironment.Rd: reticulate
  setRowNames.Rd: SingleCellExperiment-class
  setSCTKDisplayRow.Rd: SingleCellExperiment-class
  singleCellTK.Rd: SingleCellExperiment-class
  subsetSCECols.Rd: SingleCellExperiment-class
  subsetSCERows.Rd: SingleCellExperiment-class, altExp
  summarizeSCE.Rd: SingleCellExperiment-class
Please provide package anchors for all Rd \link{} targets not in the
package itself and the base packages.
* checking for missing documentation entries ... OK
* checking for code/documentation mismatches ... OK
* checking Rd \usage sections ... OK
* checking Rd contents ... OK
* checking for unstated dependencies in examples ... OK
* checking contents of ‘data’ directory ... OK
* checking data for non-ASCII characters ... OK
* checking data for ASCII and uncompressed saves ... OK
* checking R/sysdata.rda ... OK
* checking files in ‘vignettes’ ... OK
* checking examples ... WARNING
Found the following significant warnings:

  Warning in .local(x, ...) : 'normalizeCounts' is deprecated.
  Warning in .local(x, ...) : 'normalizeCounts' is deprecated.
  Warning in .local(x, ...) : 'pairwiseWilcox' is deprecated.
  Warning in .local(x, ...) : 'pairwiseWilcox' is deprecated.
  Warning in .local(x, ...) : 'pairwiseWilcox' is deprecated.
  Warning in .local(x, ...) : 'aggregateAcrossCells' is deprecated.
  Warning in .local(x, ...) : 'normalizeCounts' is deprecated.
  Warning in .local(x, ...) : 'normalizeCounts' is deprecated.
  Warning in .local(x, ...) : 'aggregateAcrossCells' is deprecated.
  Warning in .local(x, ...) : 'aggregateAcrossCells' is deprecated.
  Warning in .local(x, ...) : 'normalizeCounts' is deprecated.
  Warning in .local(x, ...) : 'pairwiseWilcox' is deprecated.
  Warning in .local(x, ...) : 'pairwiseWilcox' is deprecated.
  Warning in .local(x, ...) : 'pairwiseWilcox' is deprecated.
  Warning in .local(x, ...) : 'normalizeCounts' is deprecated.
  Warning in .local(x, ...) : 'pairwiseWilcox' is deprecated.
  Warning in .local(x, ...) : 'normalizeCounts' is deprecated.
  Warning in .local(x, ...) : 'normalizeCounts' is deprecated.
  Warning in .local(x, ...) : 'pairwiseWilcox' is deprecated.
  Warning in .local(x, ...) : 'pairwiseWilcox' is deprecated.
  Warning in .local(x, ...) : 'normalizeCounts' is deprecated.
  Warning in .local(x, ...) : 'normalizeCounts' is deprecated.
  Warning in .local(x, ...) : 'normalizeCounts' is deprecated.
  Warning in .local(x, ...) : 'librarySizeFactors' is deprecated.
  Warning in .local(x, ...) : 'normalizeCounts' is deprecated.
  Warning in .local(x, ...) : 'aggregateAcrossCells' is deprecated.
  Warning in .local(x, ...) : 'aggregateAcrossCells' is deprecated.
  Warning in .local(x, ...) : 'aggregateAcrossCells' is deprecated.
  Warning in .local(x, ...) : 'aggregateAcrossCells' is deprecated.
  Warning in .local(x, ...) : 'aggregateAcrossCells' is deprecated.
  Warning in .local(x, ...) : 'aggregateAcrossCells' is deprecated.
  Warning in .local(x, ...) : 'aggregateAcrossCells' is deprecated.
  Warning in .local(x, ...) : 'aggregateAcrossCells' is deprecated.
  Warning in .local(x, ...) : 'aggregateAcrossCells' is deprecated.
  Warning in .local(x, ...) : 'aggregateAcrossCells' is deprecated.
  Warning in fitTrendVar(fm, fv, ...) : 'fitTrendVar' is deprecated.
  Warning in .local(x, ...) : 'normalizeCounts' is deprecated.
  Warning in .local(x, ...) : 'aggregateAcrossCells' is deprecated.
  Warning in .local(x, ...) : 'aggregateAcrossCells' is deprecated.
  Warning in .local(x, ...) : 'normalizeCounts' is deprecated.
  Warning in .local(x, ...) : 'normalizeCounts' is deprecated.
  Warning in .local(x, ...) : 'normalizeCounts' is deprecated.
  Warning in .local(x, ...) : 'normalizeCounts' is deprecated.
  Warning in fitTrendVar(fm, fv, ...) : 'fitTrendVar' is deprecated.
  Warning in .local(x, ...) : 'pairwiseWilcox' is deprecated.
  Warning in .local(x, ...) : 'pairwiseWilcox' is deprecated.
  Warning in .local(x, ...) : 'normalizeCounts' is deprecated.
  Warning in .local(x, ...) : 'normalizeCounts' is deprecated.
  Warning in .local(x, ...) : 'normalizeCounts' is deprecated.
  Warning in .local(x, ...) : 'normalizeCounts' is deprecated.
  Warning in .local(x, ...) : 'normalizeCounts' is deprecated.
  Warning in .local(x, ...) : 'sumCountsAcrossCells' is deprecated.
  Warning in .local(x, ...) : 'summarizeAssayByGroup' is deprecated.
  Warning in .local(x, ...) : 'normalizeCounts' is deprecated.
  Warning in fitTrendVar(fm, fv, ...) : 'fitTrendVar' is deprecated.
  Warning in .local(x, ...) : 'normalizeCounts' is deprecated.
  Warning in .local(x, ...) : 'librarySizeFactors' is deprecated.
  Warning in .local(x, ...) : 'aggregateAcrossCells' is deprecated.
  Warning in .local(x, ...) : 'aggregateAcrossCells' is deprecated.
  Warning in .local(x, ...) : 'aggregateAcrossCells' is deprecated.
  Warning in .local(x, ...) : 'normalizeCounts' is deprecated.
  Warning in .local(x, ...) : 'normalizeCounts' is deprecated.
  Warning in .local(x, ...) : 'normalizeCounts' is deprecated.
  Warning in .local(x, ...) : 'normalizeCounts' is deprecated.
  Warning in fitTrendVar(fm, fv, ...) : 'fitTrendVar' is deprecated.
  Warning in .local(x, ...) : 'normalizeCounts' is deprecated.
Deprecated functions may be defunct as soon as of the next release of
R.
See ?Deprecated.
Examples with CPU (user + system) or elapsed time > 5s
                           user system elapsed
plotDoubletFinderResults 38.058  0.378  39.947
runSeuratSCTransform     31.213  1.704  32.925
runDoubletFinder         31.755  0.188  31.945
plotScDblFinderResults   27.966  0.240  25.279
runScDblFinder           16.547  0.283  13.863
plotBatchCorrCompare     11.753  0.049  11.802
plotScdsHybridResults    10.197  0.100   9.671
importExampleData         9.714  0.345  10.508
plotDecontXResults        7.938  0.192   8.130
plotBcdsResults           7.957  0.127   7.468
runUMAP                   7.660  0.255   7.915
plotCxdsResults           6.922  0.020   6.941
plotEmptyDropsResults     6.625  0.030   6.656
plotEmptyDropsScatter     6.609  0.029   6.638
plotUMAP                  6.490  0.009   6.499
runDecontX                6.438  0.040   6.479
runEmptyDrops             6.318  0.012   6.330
detectCellOutlier         6.138  0.133   6.274
plotDEGViolin             5.346  0.059   5.399
runEnrichR                0.534  0.038  16.006
* checking for unstated dependencies in ‘tests’ ... OK
* checking tests ...
  Running ‘spelling.R’
  Running ‘testthat.R’
 OK
* checking for unstated dependencies in vignettes ... OK
* checking package vignettes ... OK
* checking re-building of vignette outputs ... OK
* checking PDF version of manual ... OK
* DONE

Status: 3 WARNINGs
See
  ‘/home/biocbuild/bbs-3.24-bioc/meat/singleCellTK.Rcheck/00check.log’
for details.


Installation output

singleCellTK.Rcheck/00install.out

##############################################################################
##############################################################################
###
### Running command:
###
###   /home/biocbuild/bbs-3.24-bioc/R/bin/R CMD INSTALL singleCellTK
###
##############################################################################
##############################################################################


* installing to library ‘/home/biocbuild/bbs-3.24-bioc/R/site-library’
* installing *source* package ‘singleCellTK’ ...
** this is package ‘singleCellTK’ version ‘2.23.0’
** using staged installation
** R
** data
** exec
** inst
** byte-compile and prepare package for lazy loading
** help
*** installing help indices
** building package indices
** installing vignettes
** testing if installed package can be loaded from temporary location
** testing if installed package can be loaded from final location
** testing if installed package keeps a record of temporary installation path
* DONE (singleCellTK)

Tests output

singleCellTK.Rcheck/tests/spelling.Rout


R version 4.6.0 RC (2026-04-17 r89917) -- "Because it was There"
Copyright (C) 2026 The R Foundation for Statistical Computing
Platform: x86_64-pc-linux-gnu

R is free software and comes with ABSOLUTELY NO WARRANTY.
You are welcome to redistribute it under certain conditions.
Type 'license()' or 'licence()' for distribution details.

R is a collaborative project with many contributors.
Type 'contributors()' for more information and
'citation()' on how to cite R or R packages in publications.

Type 'demo()' for some demos, 'help()' for on-line help, or
'help.start()' for an HTML browser interface to help.
Type 'q()' to quit R.

> if (requireNamespace('spelling', quietly = TRUE))
+   spelling::spell_check_test(vignettes = TRUE, error = FALSE, skip_on_cran = TRUE)
All Done!
> 
> proc.time()
   user  system elapsed 
  0.158   0.042   0.183 

singleCellTK.Rcheck/tests/testthat.Rout


R version 4.6.0 RC (2026-04-17 r89917) -- "Because it was There"
Copyright (C) 2026 The R Foundation for Statistical Computing
Platform: x86_64-pc-linux-gnu

R is free software and comes with ABSOLUTELY NO WARRANTY.
You are welcome to redistribute it under certain conditions.
Type 'license()' or 'licence()' for distribution details.

R is a collaborative project with many contributors.
Type 'contributors()' for more information and
'citation()' on how to cite R or R packages in publications.

Type 'demo()' for some demos, 'help()' for on-line help, or
'help.start()' for an HTML browser interface to help.
Type 'q()' to quit R.

> library(testthat)
> library(singleCellTK)
Loading required package: SummarizedExperiment
Loading required package: MatrixGenerics
Loading required package: matrixStats

Attaching package: 'MatrixGenerics'

The following objects are masked from 'package:matrixStats':

    colAlls, colAnyNAs, colAnys, colAvgsPerRowSet, colCollapse,
    colCounts, colCummaxs, colCummins, colCumprods, colCumsums,
    colDiffs, colIQRDiffs, colIQRs, colLogSumExps, colMadDiffs,
    colMads, colMaxs, colMeans2, colMedians, colMins, colOrderStats,
    colProds, colQuantiles, colRanges, colRanks, colSdDiffs, colSds,
    colSums2, colTabulates, colVarDiffs, colVars, colWeightedMads,
    colWeightedMeans, colWeightedMedians, colWeightedSds,
    colWeightedVars, rowAlls, rowAnyNAs, rowAnys, rowAvgsPerColSet,
    rowCollapse, rowCounts, rowCummaxs, rowCummins, rowCumprods,
    rowCumsums, rowDiffs, rowIQRDiffs, rowIQRs, rowLogSumExps,
    rowMadDiffs, rowMads, rowMaxs, rowMeans2, rowMedians, rowMins,
    rowOrderStats, rowProds, rowQuantiles, rowRanges, rowRanks,
    rowSdDiffs, rowSds, rowSums2, rowTabulates, rowVarDiffs, rowVars,
    rowWeightedMads, rowWeightedMeans, rowWeightedMedians,
    rowWeightedSds, rowWeightedVars

Loading required package: GenomicRanges
Loading required package: stats4
Loading required package: BiocGenerics
Loading required package: generics

Attaching package: 'generics'

The following objects are masked from 'package:base':

    as.difftime, as.factor, as.ordered, intersect, is.element, setdiff,
    setequal, union


Attaching package: 'BiocGenerics'

The following objects are masked from 'package:stats':

    IQR, mad, sd, var, xtabs

The following objects are masked from 'package:base':

    Filter, Find, Map, Position, Reduce, anyDuplicated, aperm, append,
    as.data.frame, basename, cbind, colnames, dirname, do.call,
    duplicated, eval, evalq, get, grep, grepl, is.unsorted, lapply,
    mapply, match, mget, order, paste, pmax, pmax.int, pmin, pmin.int,
    rank, rbind, rownames, sapply, saveRDS, table, tapply, unique,
    unsplit, which.max, which.min

Loading required package: S4Vectors

Attaching package: 'S4Vectors'

The following object is masked from 'package:utils':

    findMatches

The following objects are masked from 'package:base':

    I, expand.grid, unname

Loading required package: IRanges
Loading required package: Seqinfo
Loading required package: Biobase
Welcome to Bioconductor

    Vignettes contain introductory material; view with
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Attaching package: 'Biobase'

The following object is masked from 'package:MatrixGenerics':

    rowMedians

The following objects are masked from 'package:matrixStats':

    anyMissing, rowMedians

Loading required package: SingleCellExperiment
Loading required package: DelayedArray
Loading required package: Matrix

Attaching package: 'Matrix'

The following object is masked from 'package:S4Vectors':

    expand

Loading required package: S4Arrays
Loading required package: abind

Attaching package: 'S4Arrays'

The following object is masked from 'package:abind':

    abind

The following object is masked from 'package:base':

    rowsum

Loading required package: SparseArray

Attaching package: 'DelayedArray'

The following objects are masked from 'package:base':

    apply, scale, sweep


Attaching package: 'singleCellTK'

The following object is masked from 'package:BiocGenerics':

    plotPCA

> 
> test_check("singleCellTK")
Found 2 batches
Using null model in ComBat-seq.
Adjusting for 0 covariate(s) or covariate level(s)
Estimating dispersions
Fitting the GLM model
Shrinkage off - using GLM estimates for parameters
Adjusting the data
Found 2 batches
Using null model in ComBat-seq.
Adjusting for 1 covariate(s) or covariate level(s)
Estimating dispersions
Fitting the GLM model
Shrinkage off - using GLM estimates for parameters
Adjusting the data
Performing log-normalization
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Calculating gene variances
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[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Calculating feature variances of standardized and clipped values
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[----|----|----|----|----|----|----|----|----|----|
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Calculating gene variances
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[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Calculating feature variances of standardized and clipped values
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[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Uploading data to Enrichr... Done.
  Querying HDSigDB_Human_2021... Done.
Parsing results... Done.
Performing log-normalization
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[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Calculating gene variances
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[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Calculating feature variances of standardized and clipped values
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[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Calculating gene means
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[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Calculating gene variance to mean ratios
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[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Calculating gene means
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[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Calculating gene variance to mean ratios
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[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Performing log-normalization
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[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Calculating gene variances
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[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Calculating feature variances of standardized and clipped values
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[----|----|----|----|----|----|----|----|----|----|
**************************************************|

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[04:57:36] WARNING: src/learner.cc:782: 
Parameters: { "nthreads" } are not used.

[04:57:37] WARNING: src/learner.cc:782: 
Parameters: { "nthreads" } are not used.

Performing log-normalization
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[----|----|----|----|----|----|----|----|----|----|
**************************************************|

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Calculating gene variances
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[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Calculating feature variances of standardized and clipped values
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[----|----|----|----|----|----|----|----|----|----|
**************************************************|

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Modularity Optimizer version 1.3.0 by Ludo Waltman and Nees Jan van Eck

Number of nodes: 390
Number of edges: 9849

Running Louvain algorithm...
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[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Maximum modularity in 10 random starts: 0.8351
Number of communities: 7
Elapsed time: 0 seconds
Using method 'umap'
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[----|----|----|----|----|----|----|----|----|----|
**************************************************|
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[----|----|----|----|----|----|----|----|----|----|
**************************************************|

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Performing log-normalization
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[----|----|----|----|----|----|----|----|----|----|
**************************************************|
[ FAIL 0 | WARN 84 | SKIP 0 | PASS 225 ]

[ FAIL 0 | WARN 84 | SKIP 0 | PASS 225 ]
> 
> proc.time()
   user  system elapsed 
290.125  11.814 304.247 

Example timings

singleCellTK.Rcheck/singleCellTK-Ex.timings

nameusersystemelapsed
MitoGenes0.0010.0010.002
SEG0.0030.0000.003
calcEffectSizes0.1560.0030.159
combineSCE0.7960.0990.895
computeZScore0.2970.0120.309
convertSCEToSeurat4.6900.2514.941
convertSeuratToSCE0.3090.0020.311
dedupRowNames0.0550.0010.056
detectCellOutlier6.1380.1336.274
diffAbundanceFET0.060.000.06
discreteColorPalette0.0070.0000.007
distinctColors0.0030.0000.002
downSampleCells0.5290.0210.550
downSampleDepth0.4500.0060.457
expData-ANY-character-method0.1250.0070.132
expData-set-ANY-character-CharacterOrNullOrMissing-logical-method0.1660.0050.170
expData-set0.1540.0090.163
expData0.1380.0020.140
expDataNames-ANY-method0.1290.0000.129
expDataNames0.1250.0000.126
expDeleteDataTag0.0360.0000.037
expSetDataTag0.0250.0000.025
expTaggedData0.0260.0000.026
exportSCE0.0220.0000.021
exportSCEtoAnnData0.0900.0090.099
exportSCEtoFlatFile0.0940.0050.099
featureIndex0.0370.0030.040
generateSimulatedData0.0560.0020.057
getBiomarker0.0650.0010.066
getDEGTopTable0.6970.0750.772
getDiffAbundanceResults0.0480.0000.048
getEnrichRResult0.6300.0373.797
getFindMarkerTopTable1.5580.0041.562
getMSigDBTable0.0030.0000.004
getPathwayResultNames0.0220.0000.022
getSampleSummaryStatsTable0.2780.0000.277
getSoupX0.0010.0000.000
getTSCANResults1.1260.0091.135
getTopHVG0.7530.0020.754
importAnnData0.0020.0000.002
importBUStools0.1550.0000.156
importCellRanger0.7640.0060.771
importCellRangerV2Sample0.1480.0000.148
importCellRangerV3Sample0.2940.0030.296
importDropEst0.1940.0020.196
importExampleData 9.714 0.34510.508
importGeneSetsFromCollection0.0750.0000.076
importGeneSetsFromGMT0.0610.0010.062
importGeneSetsFromList0.1210.0000.121
importGeneSetsFromMSigDB2.1440.0742.218
importMitoGeneSet0.0540.0010.055
importOptimus0.0020.0000.001
importSEQC0.1160.0000.116
importSTARsolo0.1390.0020.141
iterateSimulations0.1600.0020.162
listSampleSummaryStatsTables0.2760.0020.278
mergeSCEColData0.3280.0030.331
mouseBrainSubsetSCE0.0350.0010.037
msigdb_table0.0010.0010.002
plotBarcodeRankDropsResults0.9030.0100.913
plotBarcodeRankScatter0.7980.0000.798
plotBatchCorrCompare11.753 0.04911.802
plotBatchVariance0.4210.0840.506
plotBcdsResults7.9570.1277.468
plotBubble0.7850.0020.788
plotClusterAbundance1.2880.0041.293
plotCxdsResults6.9220.0206.941
plotDEGHeatmap2.2080.0982.306
plotDEGRegression4.4420.0494.484
plotDEGViolin5.3460.0595.399
plotDEGVolcano0.9680.0180.985
plotDecontXResults7.9380.1928.130
plotDimRed0.2720.0010.274
plotDoubletFinderResults38.058 0.37839.947
plotEmptyDropsResults6.6250.0306.656
plotEmptyDropsScatter6.6090.0296.638
plotFindMarkerHeatmap3.6440.0493.693
plotMASTThresholdGenes1.1660.0111.177
plotPCA0.3500.0030.353
plotPathway0.6330.0010.635
plotRunPerCellQCResults2.9420.0743.016
plotSCEBarAssayData0.2700.0010.270
plotSCEBarColData0.2240.0010.224
plotSCEBatchFeatureMean0.3750.0030.378
plotSCEDensity0.3020.0000.302
plotSCEDensityAssayData0.280.000.28
plotSCEDensityColData0.2840.0010.285
plotSCEDimReduceColData0.7050.0010.706
plotSCEDimReduceFeatures0.3600.0020.363
plotSCEHeatmap0.4240.0010.425
plotSCEScatter0.3300.0010.331
plotSCEViolin0.3370.0000.337
plotSCEViolinAssayData0.3590.0010.360
plotSCEViolinColData0.3660.0000.366
plotScDblFinderResults27.966 0.24025.279
plotScanpyDotPlot0.0210.0000.021
plotScanpyEmbedding0.0200.0010.021
plotScanpyHVG0.0220.0000.021
plotScanpyHeatmap0.0210.0000.021
plotScanpyMarkerGenes0.0210.0000.021
plotScanpyMarkerGenesDotPlot0.0210.0000.021
plotScanpyMarkerGenesHeatmap0.0200.0000.021
plotScanpyMarkerGenesMatrixPlot0.0200.0010.021
plotScanpyMarkerGenesViolin0.0210.0000.021
plotScanpyMatrixPlot0.0210.0000.021
plotScanpyPCA0.0210.0000.021
plotScanpyPCAGeneRanking0.0200.0000.021
plotScanpyPCAVariance0.0210.0000.021
plotScanpyViolin0.0210.0000.021
plotScdsHybridResults10.197 0.100 9.671
plotScrubletResults0.0220.0000.022
plotSeuratElbow0.0220.0000.021
plotSeuratHVG0.0200.0010.021
plotSeuratJackStraw0.0200.0010.022
plotSeuratReduction0.0210.0010.021
plotSoupXResults000
plotTSCANClusterDEG4.5990.0034.602
plotTSCANClusterPseudo1.2900.0011.292
plotTSCANDimReduceFeatures1.2870.0011.289
plotTSCANPseudotimeGenes1.5380.0051.543
plotTSCANPseudotimeHeatmap1.2890.0061.295
plotTSCANResults1.1850.0041.188
plotTSNE0.3570.0000.357
plotTopHVG0.6440.0070.651
plotUMAP6.4900.0096.499
readSingleCellMatrix0.0050.0000.006
reportCellQC0.080.000.08
reportDropletQC0.0230.0000.023
reportQCTool0.0810.0000.081
retrieveSCEIndex0.0280.0000.027
runBBKNN000
runBarcodeRankDrops0.2180.0000.218
runBcds1.4870.0690.976
runCellQC0.0780.0010.079
runClusterSummaryMetrics0.3670.0030.370
runComBatSeq0.4430.0110.454
runCxds0.2990.0020.301
runCxdsBcdsHybrid1.4690.0540.950
runDEAnalysis0.3590.0000.360
runDecontX6.4380.0406.479
runDimReduce0.2740.0010.275
runDoubletFinder31.755 0.18831.945
runDropletQC0.0220.0000.021
runEmptyDrops6.3180.0126.330
runEnrichR 0.534 0.03816.006
runFastMNN1.6410.0411.682
runFeatureSelection0.2090.0020.211
runFindMarker1.4400.0031.443
runGSVA0.6500.0130.663
runHarmony0.0390.0020.041
runKMeans0.2020.0200.222
runLimmaBC0.0790.0010.080
runMNNCorrect0.3760.0150.391
runModelGeneVar0.2760.0070.284
runNormalization2.2060.0442.250
runPerCellQC0.3270.0020.328
runSCANORAMA000
runSCMerge0.0040.0000.004
runScDblFinder16.547 0.28313.863
runScanpyFindClusters0.0210.0010.022
runScanpyFindHVG0.0210.0010.022
runScanpyFindMarkers0.0210.0000.022
runScanpyNormalizeData0.0940.0000.094
runScanpyPCA0.0210.0000.021
runScanpyScaleData0.0210.0000.021
runScanpyTSNE0.020.000.02
runScanpyUMAP0.0210.0000.021
runScranSNN0.2900.0020.292
runScrublet0.0230.0000.023
runSeuratFindClusters0.0220.0000.022
runSeuratFindHVG0.4660.0010.467
runSeuratHeatmap0.0210.0010.023
runSeuratICA0.0220.0000.022
runSeuratJackStraw0.0220.0000.021
runSeuratNormalizeData0.0210.0000.022
runSeuratPCA0.0220.0000.022
runSeuratSCTransform31.213 1.70432.925
runSeuratScaleData0.0240.0020.026
runSeuratUMAP0.0220.0020.023
runSingleR0.0400.0040.043
runSoupX000
runTSCAN0.7360.0280.764
runTSCANClusterDEAnalysis0.8590.0750.934
runTSCANDEG0.7850.0140.799
runTSNE0.7380.0070.745
runUMAP7.6600.2557.915
runVAM0.3110.0010.312
runZINBWaVE0.0040.0000.004
sampleSummaryStats0.1790.0010.180
scaterCPM0.1600.0070.167
scaterPCA0.4670.0100.477
scaterlogNormCounts0.2480.0150.264
sce0.0220.0010.023
sctkListGeneSetCollections0.0860.0010.087
sctkPythonInstallConda0.0000.0000.001
sctkPythonInstallVirtualEnv000
selectSCTKConda000
selectSCTKVirtualEnvironment000
setRowNames0.0900.0010.091
setSCTKDisplayRow0.4410.0180.459
singleCellTK000
subDiffEx0.3220.0070.329
subsetSCECols0.0840.0020.086
subsetSCERows0.2330.0240.257
summarizeSCE0.0730.0040.076
trimCounts0.2200.0060.226