Documentation updates
- In previous versions, the functionality of the
uniq_pep
parameter (see create_df help page) was incorrectly
described. We corrected the description to match its functionality as
follows: “Numerical. Proteins that are identified by this number or
fewer number of unique peptides are filtered out (default is 2).Only
applies when input_type = "MaxQuant".
Bug fixes
- In previous versions,
volcano_plot function plotted
“-log10(P-value)” on the y-axis by default even when
sig = "adjP" and only colored the dots by their
significance based on “adjP”. In this version, sig = "adjP"
shows “-log10.adj. P-value” on the y-axis and colors the dots
by their “adjP” significance.
New data types allowed
- A new argument (
data_type) added to the
create_df function to accommodate other types of LFQ data
(raw intensity, iBAQ).
- A new argument
input_type added to the
create_df function to allow users to input data from a
standard quantitative matrix.
Workflow changes
- To allow for missing data imputation prior to or after data
normalization step (depending on the imputation method used), the
following changes were made:
norm_df and imp_df arguments replaced with
a generic df argument in the functions,
find_dep, impute_na,
normalize_data, and heatmap_de
- A note was added to the tutorials to clarify that for some
imputation methods, such as the kNN method, data normalization should be
performed prior to imputation.
Default machine learning
algorithms
naive_bayes added to the default
algorithm_list argument in the train_models
function.
Bug fixes
- Fixes a minor issue with
create_df when removing
potential contaminants. The number of potential contaminants removed is
now shown in the console.
- Fixes an issue with
find_dep that previously used a
fixed value for the adj_method argument.
- Fixes an issue with the
file_path argument for saving
the “TopHits.txt” file produced by the find_dep
function.
Other changes
- Citation file updated with the biorxiv preprint details.
- Readme file updated with information on the Shiny App.
- Help pages updated.