To install this package, start R and enter:
## try http:// if https:// URLs are not supported
source("https://bioconductor.org/biocLite.R")
biocLite("ddCt")
In most cases, you don't need to download the package archive at all.
ddCt
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This package is for version 3.4 of Bioconductor; for the stable, up-to-date release version, see ddCt.
The ddCt Algorithm for the Analysis of Quantitative Real-Time PCR (qRT-PCR)
Bioconductor version: 3.4
The Delta-Delta-Ct (ddCt) Algorithm is an approximation method to determine relative gene expression with quantitative real-time PCR (qRT-PCR) experiments. Compared to other approaches, it requires no standard curve for each primer-target pair, therefore reducing the working load and yet returning accurate enough results as long as the assumptions of the amplification efficiency hold. The ddCt package implements a pipeline to collect, analyse and visualize qRT-PCR results, for example those from TaqMan SDM software, mainly using the ddCt method. The pipeline can be either invoked by a script in command-line or through the API consisting of S4-Classes, methods and functions.
Author: Jitao David Zhang, Rudolf Biczok, and Markus Ruschhaupt
Maintainer: Jitao David Zhang <jitao_david.zhang at roche.com>
Citation (from within R,
enter citation("ddCt")):
Installation
To install this package, start R and enter:
## try http:// if https:// URLs are not supported
source("https://bioconductor.org/biocLite.R")
biocLite("ddCt")
Documentation
To view documentation for the version of this package installed in your system, start R and enter:
browseVignettes("ddCt")
| R Script | Analyse RT-PCR data with the end-to-end script in ddCt package | |
| R Script | How to apply the ddCt method | |
| R Script | Introduction to the ddCt method for qRT-PCR data analysis: background, algorithm and example | |
| Reference Manual | ||
| Text | NEWS |
Details
| biocViews | DifferentialExpression, GeneExpression, MicrotitrePlateAssay, Software, qPCR |
| Version | 1.30.0 |
| In Bioconductor since | BioC 2.5 (R-2.10) (7.5 years) |
| License | LGPL-3 |
| Depends | R (>= 2.3.0), methods |
| Imports | Biobase(>= 1.10.0), RColorBrewer (>= 0.1-3), xtable, lattice, BiocGenerics |
| LinkingTo | |
| Suggests | RUnit |
| SystemRequirements | |
| Enhances | |
| URL | |
| Depends On Me | |
| Imports Me | |
| Suggests Me | |
| Build Report |
Package Archives
Follow Installation instructions to use this package in your R session.
| Package Source | ddCt_1.30.0.tar.gz |
| Windows Binary | ddCt_1.30.0.zip |
| Mac OS X 10.9 (Mavericks) | ddCt_1.30.0.tgz |
| Subversion source | (username/password: readonly) |
| Git source | https://github.com/Bioconductor-mirror/ddCt/tree/release-3.4 |
| Package Short Url | http://bioconductor.org/packages/ddCt/ |
| Package Downloads Report | Download Stats |
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