iscream depends on htslib header files and uses Rhtslib as the source by default. However, using htslib from other sources can improve its performance. See https://huishenlab.github.io/iscream/dev/articles/htslib.html. Ubuntu: sudo apt-get install -y libhts-dev tabix Fedora/RHEL: sudo dnf install -y htslib-devel MacOS with brew: brew install htslib